R&D (Research & Development)

Intergen is a center established with the aim of providing patient services and conducting research and development. We have ensured that all types of genetic tests are performed in our center using over 90% in-house produced materials and methods.

Research and Development (R&D) at Intergen

 

Founded in 1999 with the motto “People, Health, and Science,” Intergen is a center established with the goal of providing patient services and conducting research and development.

In the field of rare diseases, it is often necessary to develop custom materials and test methods for each patient and even to discover and define new diseases.

 

What Have We Accomplished at the Intergen R&D Center?

 

  • We eliminated dependency on foreign countries for diagnostics.

  • We ensured that all types of genetic tests are conducted within our center using over 90% in-house produced materials and methods.

  • We reduced reliance in the field of novel targeted therapies.

With our academically accomplished team members:

  • We are currently conducting over 45 projects with our own internal resources,

  • Receiving additional support from institutions such as TÜBİTAK,

  • Advancing through university and industry collaborations,

  • And making strides both in fundamental science and patient services.

 

Intergen R&D Center:

 

  • With over 60,000 designed primers and proprietary methods,

  • We use our own designs in 90% of the thousands of genetic tests conducted for over 5,500 diseases.

  • Instead of saying “BUT” during the planning phase of scientific collaborations, we say “HOW ABOUT” and actively engage with our young team.

  • We support inexperienced young researchers so they too can contribute to our country.

  • Our R&D activities are not only aimed at providing the highest quality healthcare services to our patients but also at representing our country abroad with high value-added products.

  • We continuously increase our R&D budget to invest in people and knowledge.

  • We don’t just follow emerging technologies—we help shape them.

 

Services Offered by the Intergen R&D Center

 

We support projects conducted by other institutions using nearly all genetic testing techniques, especially the following:

 

1. Whole Exome Sequencing (WES)

Whole Exome Sequencing is one of the most comprehensive methods for diagnosing genetic disorders. This method sequences all coding regions of the ~20,000 genes in the human genome to detect disease-associated variations. It is ideal when the target gene regions are unclear or too numerous, offering fast, reliable, and accessible results.

This method is also used in our center for research involving non-human organisms, provided that a commercial kit is available.

 

2. Whole Genome Sequencing (WGS)

In certain cases, diagnosis of a genetic disorder requires sequencing the entire genome—approximately 3 billion base pairs. The resulting analysis helps clarify the patient’s clinical condition.

This method is also applied in our center for non-human organism studies.

 

3. Sanger Sequencing

Regarded as the gold standard of sequencing technologies, Sanger sequencing is used in all phases of our scientific research—from experimental design to result analysis. With our instrumentation and in-house solutions, we contribute significantly to science in Turkey.

All stages, from PCR (the starting point) to capillary electrophoresis and data analysis with our bioinformatics team, are carried out within our center.

In addition to Sanger sequencing, fragment analysis, genotyping, and quantitative studies are used in our R&D projects.

This method is also available for non-human organisms.
Project-specific materials are designed.

 

4. Next Generation Sequencing (NGS)

NGS technologies allow for high-throughput sequencing and represent a revolutionary advance. At Intergen, we utilize different sequencing scales in our projects based on their size and scope.

This method is used for non-human organisms as well.
Project-specific materials are designed.

 

5. Methylation Studies

For genes affected by methylation, we provide support through methylation-specific PCR, array-based, and methylome studies.

This method is used in research involving non-human organisms.
Project-specific materials are designed.

 

6. Real-time PCR Applications (TaqMan, SYBR Green)

  • Genotyping

  • Gene expression analysis

This method is used in non-human organism research.
Project-specific materials are designed.

 

7. Cloning

  • Vector design

  • TA cloning

  • Gibson cloning

 

8. Cell-Free Protein Synthesis

This approach enables protein expression without living cells using plasmid DNA, linear DNA, or mRNA templates.

This method is also used for non-human organisms in our center.

 

9. Design and Production of Laboratory Equipment with 3D Printing

We modify and customize plastic lab equipment for internal use at our center.

Project-specific designs can be created. Please contact us.

 

10. Training and Workshops

  • Next Generation Sequencing (NGS) Workshop

  • Sanger Sequencing Workshop

  • Primer and Probe Design Workshop

  • PCR, Real-time PCR, and Gene Expression Workshop

  • Fluorescence In Situ Hybridization (FISH) Workshop

 

11. Primer, Probe, and Oligo Design